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KMID : 0894520070110030167
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Development & Reproduction
2007 Volume.11 No. 3 p.167 ~ p.177
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Characterization Analysis for Cardiogenic Potential of Three Human Adult Stem Cells
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Park Se-Ah
Kang Hyun-Mi
Kim Eun-Su
Kim Jin-Young
Kim Hae-kwon
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Abstract
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In the present study, we isolated three human adult stem cells including adipose tissue-derived stem cells(HAD), umbilical cord-derived stem cells(HUC), and amnion-derived stem cells(HAM) and analysed their characteristics. And we examined whether HAD could be used as therapeutical cells for the heart diseases.
Both HAM and HUC appeared very similar morphology but HAD was different. Doubling time of HUC was most fast, but total doubling numbers of HUC was same with HAM. Total doubling numbers of HAD was much more than others. Expression patterns of genes and proteins of three human adult stem cells were very similar. Also they were differentiated into adipocytes, osteocytes, and chondrocytes. In addition, they expressed many cardiomyocyte-related genes. But expression pattern of genes is a little different. When HAD were cultivated in the presence or absence of various combinations of BMP and FGF after 5-azacytidine expose for 24 h, expression of Cmlc-1, and α1c genes was significantly increased. However, expression of troponin T, troponin I and Kv4.3 genes was not changed. Based on these observations, it is suggested that HAD, HUC, and HAM might be used as potentially therapeutical cells for clinical application.
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KEYWORD
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Mesenchymal stem cells, Adipose tissue-derived stem cells, Umbilical cord-derived stem cells, Amnion-derived stem cells, Clinical application
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